an elispot based assay to measure hbv specific cd8 t cell responses in immunocompetent mice
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
Ngày tải lên :
24/12/2016, 22:30
... enterohepatic circulation of BAs [3] The NTCP transport activity is regulated at both the transcriptional [4] and the posttranslational level Posttranslational regulation of NTCP transport activity ... progenitor cells bearing the potential to differentiate into hepatocyte-like granular cells and biliary cells following DMSO treatment [8] In addition to their susceptibility to HBV and Hepatitis ... pharmacological and toxicological studies besides in vitro HBV and HDV infection/coinfection Interestingly, it turns out that sodium taurocholate cotransporting polypeptide (NTCP), one of the transporters...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
Ngày tải lên :
16/03/2014, 04:20
... and listed in previous reports [22–24], except for SIRT1 (sense primer 5¢-CTGTTTTTACCACCAAATCG-3¢ and antisense primer 5¢-CAACTTGTTGCTTGTTGGAT-3¢) and SIRT2 (sense primer 5¢-ATGTCCCTCATGGGCTTCGG-3¢ ... PMA/ionomycin treatment of DT40 cells resulted in cell- cycle distributions that were quite different from those in the case of the etoposide treatment, it did induce apoptosis, and these findings agree with ... expected, etoposide treatment caused apoptotic cell death for the two cell lines at the same level because the drug inhibits the topoisomerase-2 activity that is essential for DNA replication Although...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
Ngày tải lên :
22/03/2014, 21:21
... recombinant protein from L of medium for all three mutants Activation and further purification of the recombinant HC mutants Following the initial Ni2+–nitrilotriacetic acid agarose purification, the three ... Andersson et al P2¢ specificity of the human mast cell chymase Results Production and purification of recombinant HC mutants Two single mutants of HC, Arg143 fi Gln and Lys192 fi Met, and a double mutant, ... 5¢-CCAGAGTCTCCCATAAATGCAGA TTTTGTCTTCCTG-3¢ These primers had a melting temperature of 78 °C, and the mismatches resulting in replacement of the Lys codon with a Met codon are underlined The Arg143...
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Báo cáo y học: "Specific eradication of HIV-1 from infected cultured cells" doc
Ngày tải lên :
10/08/2014, 05:21
... contributed to writing the paper; ZH performed peptide synthesis and purification; AF designed the study, and contributed to the writing; A Loyter designed the study, contributed to the writing ... of the paper and coordinated the study All authors have read and approved the manuscript Figure Correlation between cell death and treatment H9 lymphocyte T cells were infected with the WT HIV-1 ... weeks treatment, cells were left to grow untreated (a) The average amount of viral RNA copies per cell was estimated as described in [38]: prior to treatment, at the end of the two weeks treatments...
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Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury" ppt
Ngày tải lên :
18/06/2014, 19:20
... security Therefore, it is important to find new ways to modify antioxidant enzyme for the efficient introduction into cells Huang et al Journal of Translational Medicine 2011, 9:73 http://www.translational-medicine.com/content/9/1/73 ... PEP-1-CAT group n = The antioxidant enzymes (SOD1 and CAT) have the potential to prevent OFR-mediated tissue damage, but they cannot freely pass the cell membrane, which limits their applications ... B, C) The wild type SOD1 or CAT was not transduced into the cells (Figure 2) It is essential that transduced PEP-1-SOD1 or PEP-1CAT fusion proteins in cells retain their enzymatic activity Therefore,...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
Ngày tải lên :
18/02/2014, 08:20
... inability to see potentiation with nonsteroidal compounds at least demonstrates that the effects of JHIII cannot be attributed to a generalized cellular action upon the transcriptional complex that includes ... ATC-3¢ and the reverse primer TCTAGA CTA TGT CTT CAT GTC GAC 5¢-TTTT GTC-3¢ The underlined portions of the primers represent the inserted BamHI and XbaI restriction sites, respectively The vector pcDNA3.1+ ... further suggest that USP plays a role in determining the quantitative level of transcriptional activity Implications for a mechanism of potentiation As noted earlier, the effects of potentiation...
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Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
Ngày tải lên :
23/03/2014, 10:20
... using the following primers: DmPCNA2 ) forward, 5¢-ATGCTCGA GGCGCGTTTGAG-3¢, and reverse, 5¢-CTAGAAATC GGGGTCATTCA-3¢; DmPCNA1 – forward, 5¢-ATGTTC GAGGCACGCCT-3¢, and reverse, 5¢-TTATGTCTCGTT GT ... treatment (Fig 6C) In contrast, the amount of DmPCNA1 in this fraction continued to increase up to h after MMS treatment and h after H2O2 treatment (Fig 6C) UV light treatment increased the level ... factors DREF and early region transcription factor (E2F) [9,21] These transcription factors regulate the expression of DNA replication- and cell proliferation-related genes To investigate the...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
Ngày tải lên :
18/06/2014, 15:20
... Interestingly, we noticed that these receptors are linked to some extent to T cell proliferation as anti-CD3/CD28 activated T cells have a significantly lower Kd than resting autologous T cells ... NK cells initially-obtained their name due to their natural cytotoxicity against tumor cells requiring no prior sensitization, unlike T cells [4] It is well established that the cytotoxicity ... inhibits T cell activation, their maintenance, and consequently favours the representation and then further expansion of NK cells driven by IL-2 Regulatory activity of 3a-G1 is direct and does not...
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báo cáo hóa học: " Quality of life among adolescents with sickle cell disease: mediation of pain by internalizing symptoms and parenting stress" pdf
Ngày tải lên :
18/06/2014, 19:20
... the study investigators and trained research assistants (teams of doctoral students and advanced undergraduates) The protocol was approved by the appropriate Institutional Review Board Data Analysis ... complications and treatment is integral to adolescent adaptation to SCD in the context of pain The role of the family in adaptive outcomes for youth with SCD has been highlighted in the literature.[36,37] ... psychological adaptation Caregiver and teen report of pain and HRQOL and the examination of teen and family concomitant factors represent research design improvements that better inform this effort to explore...
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Báo cáo khoa học " Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris " doc
Ngày tải lên :
28/06/2014, 11:20
... transcriptase Total RNA (1 lg) was incubated with oligo-dT15 for at 70 °C, then mixed with a 5Â firststrand buffer, 10 mM dNTPs, and 0.1 M DTT The reaction mixture was further incubated for at ... microliters of the MTT stock solution (2 mg/ml) was then added to each well to attain a total reaction volume of 200 ll After incubation for h, the plate was centrifuged at 800g for and the supernatants ... conformation (Fig 4) There are some data suggesting that polysaccharides with no triple-helical conformation show great antitumor activity The antitumor activity of a (1 ? 3)-b-glucan with a high...
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Báo cáo y học: " Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" docx
Ngày tải lên :
09/08/2014, 01:22
... cell- cell interaction in the rheumatoid synovium The essential factor in this situation appears to be the T cell- monocyte interaction to the extent that T- cell isolation renders the cells sensitive ... analysis, and interpretation of data, and drafted the manuscript SR designed, performed, and analyzed the transwell experiments and participated in interpretation of the data and writing of the ... number not for citation purposes) Soluble factor(s) rather than cellular interaction are essential for the induction of the T- cell apoptosis-resistant phenotype To further investigate the mechanism...
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Báo cáo y học: "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls" docx
Ngày tải lên :
09/08/2014, 01:23
... moiety protects the peptidoglycan moiety from degradation in vivo, thereby facilitating chronic persistence of PG-PS in the host [2] The primary events and the identity of cellular receptors that ... that mediate the innate immune response to and the arthritogenic properties of peptidoglycan and PG-PS are incompletely characterized There is substantial evidence that the transmembrane signal ... TLR-2induced cell activation, but the role of CD14 is controversial On the one hand, neutralizing antibody to CD14 prevented peptidoglycan-induced cell activation, and transfection of CD14-negative cells...
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báo cáo khoa học: "A Review of Duodenal Metastases from Squamous Cell Carcinoma of the Cervix Presenting as an Upper Gastrointestinal Bleed" potx
Ngày tải lên :
09/08/2014, 02:21
... mechanical trauma Additionally, transit through the small intestine is more rapid than through the large intestine, thereby shortening the exposure of potential carcinogens in the chyme to the ... surrounding the aorta most consistent with metastatic lympadenopathy 1c: CT scan also shows evidence of some lytic destruction of the left lateral aspect of the L5 vertebral body most likely related to ... best practice therapeutic decisions in these patients Conflict of Interest: The authors declare no conflicts of interest Author Contributions: All authors participated fully in the conception,...
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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf
Ngày tải lên :
09/08/2014, 09:21
... (TOYOBO, Japan) with the following primers: hTP53AF (5’ccattcttttcctgctccacaggaagccga-3’) and hTP53BR (5’ggctaagctatgatgttccttagattaggt-3’) for exons - 9, hTP53CF (5’-ctgtataggtacttgaagtgcagtttctac ... acid substitution of proline 72 to arginine (Figure 1) Since the substitution has not been reported to confer any dominant negative effects of the gene [30], we estimated that this mutation hardly ... bars indicate standard errors of the mean of three independent experiments cisplatin did not reveal strong cytotoxic effects for the particular cell lines under the treatment condition (1 hour,...
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Báo cáo y học: "Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" pdf
Ngày tải lên :
09/08/2014, 13:22
... cell- cell interaction in the rheumatoid synovium The essential factor in this situation appears to be the T cell- monocyte interaction to the extent that T- cell isolation renders the cells sensitive ... analysis, and interpretation of data, and drafted the manuscript SR designed, performed, and analyzed the transwell experiments and participated in interpretation of the data and writing of the ... number not for citation purposes) Soluble factor(s) rather than cellular interaction are essential for the induction of the T- cell apoptosis-resistant phenotype To further investigate the mechanism...
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báo cáo khoa học: "Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I" pps
Ngày tải lên :
10/08/2014, 10:21
... method In brief, mouse anti-human Lewis y antibody and rabbit anti-human CD44 antibody were diluted to 1:100 as primary antibody solutions; goat anti-rabbit TRITC-labeled secondary antibody and ... 107 cells) to extract total RNA The concentration and purity of RNA were detected by an ultraviolet spectrometer cDNA was synthesized according to the RNA reverse transcription kit instructions ... RMG-I and RMG-I-H cells detected by immunocytochemistry (×400) Panels and are negative controls; panels and are Lewis y antibody-untreated cells; panels and are Lewis y antibody-treated cells; panels...
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báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt
Ngày tải lên :
11/08/2014, 18:20
... thousands) estimated to be in the total injection volumes, prepared without a tablet (control) or with cold or hot extraction Upper panel, unfiltered; lower panel, cigarette filtrate Total injection ... the wet filters (Table 1) The mean morphine content of the filtrate was 32 mg, which is the amount expected to be in this fraction (1.7/3.0) of the total volume of extract This indicates that ... recovery by Mann-Whitney U test (a non-parametric analogue of the t- test), 2-tailed Results are consistent with parametric analysis with Games-Howell post-hoc tests to inject Pharmaceutical standards...
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Báo cáo y học: " Analysis of Prototype Foamy Virus particle-host cell interaction with autofluorescent retroviral particles" ppt
Ngày tải lên :
12/08/2014, 23:23
... particle incubation with the target cells (Fig 8D) Taken together, these data suggest that both cell lines still demonstrate a FV Env specific attachment of retroviral particles, and a currently ... previous reports for other retroviruses [35] Target cell attachment A Identification of cell lines resistant to PFV-Env mediated vector transduction Previous attempts to identify cell lines non-permissive ... combination with high-titer FV Env containing retroviral vector supernatants, it was used to identify two cell lines that are resistant to PFV Env-mediated marker gene transfer Interestingly, these...
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Báo cáo y học: "Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice" pptx
Ngày tải lên :
13/08/2014, 13:20
... able to adoptively transfer non-tolerant T cells into a transgenic mice expressing HCV transgene in hepatocytes The transfer results in rapid and selective accumulation of the activated T cells ... The percentage of CD4+ and CD8+ T cells in the lymph nodes of the recipient mice The cells were surface stained with anti-CD3+ and anti-CD4+ antibodies or anti-CD3+ and anti -CD8+ and analyzed ... mice were to weeks old; wild type mice and the recipient mice, both HCV transgenic and non-transgenic mice, were to months old The establishment and characterization of these HCV transgenic mice...
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